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Acid-Fast Staining Example
Acid-Fast Staining Example. Procedure of acid fast stain. Ehrlich observed that dyes were not absorbed by mycobacterium unless the organism on the slide was heated.

Some of the sample is placed on a glass slide, stained, and heated. Remove the stained paper towel gently. Place the slide over a heating beaker of water.
A Slide Is Cleaned Properly Under Tap Water, Such That Water Does Not Remain As Drops On Its Surface (Figure 5.12).
Over the smear, pour and flood the smear with. The cells in the sample hold onto the dye. Some of the sample is placed on a glass slide, stained, and heated.
Clinically, The Most Important Application Is To Detect Mycobacterium Tuberculosis In Sputum Samples To Confirm Or Rule Out A Diagnosis Of Tuberculosis In Patients.
Ehrlich observed that dyes were not absorbed by mycobacterium unless the organism on the slide was heated. 2.) fix the smear onto the slide by passing over the flame of spirit lamp or bunsen burner. Albert's stains gram staining the most commonly.
Place The Slide Over A Heating Beaker Of Water.
Once the dye was absorbed, the cell wall retained the dye even when the smear was washed with a mixture of acid and alcohol (3% hydrochloric acid and 95% ethanol). Basic fuchsin is commonly used. Procedure of acid fast stain.
The Reagent Should Be Added Drop By Drop Until Carbol Fuchsin Fails To Wash From Smear.
Differentiating two different types of cell. Remove the stained paper towel gently. Acid fast staining method steps.
Prepare And Fix The Specimen Smear Prior To Staining.
The slide is then washed with an acid solution and a different stain is applied. For example, clostridium difficile is detected by the presence of a toxin in the. Procedure of acid fast staining.
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